| Product Name | NALP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | NALP1 (monoclonal Nalpy1-4) antibody was raised against recombinant human NALP1 (NACHT-, LRR- and PYD-) containing protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICCIHCIPWB |
| NALP1 can be used to recognize the pyrin domain (PYD) of human NALP1 by Immunocytochemistry (1:1,000), Immunohistochemistry (frozen sections, paraffin sections (microwave treatment)), Immunoprecipitation, and Western Blot. Method: Assembly of the inflammasome was induced by shifting the temperature to 30˚C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated with PAb to Asc (AL177) and run on SDS-PAGE. NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5,000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon Mol. Cell. 10, 417 (2002). Method: 293T cells transfected with full length human NALP1 were fixed with 4% paraformaldehyde and stained with 1 μg/mL MAb to NALP1 (Nalpy1-4) and then Alexa 488 (1:300 dilution). This product is for research use only. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | NLRP1 |
|---|---|
| Alternative Names | CARD7 DEFCAP KIAA0926 NAC NALP1 NACHT LRR and PYD domains-containing protein 1 Caspase recruitment domain-containing protein 7 CARD7 CIDED SLEV1 PP1044 VAMAS1 CLR17.1 DEFCAP-L/S |
| Application Notes | NALP1 can be used to recognize the pyrin domain (PYD) of human NALP1 by Immunocytochemistry (1:1,000), Immunohistochemistry (frozen sections, paraffin sections (microwave treatment)), Immunoprecipitation, and Western Blot. Method: Assembly of the inflammasome was induced by shifting the temperature to 30˚C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated with PAb to Asc (AL177) and run on SDS-PAGE. NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5,000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon Mol. Cell. 10, 417 (2002). Method: 293T cells transfected with full length human NALP1 were fixed with 4% paraformaldehyde and stained with 1 μg/mL MAb to NALP1 (Nalpy1-4) and then Alexa 488 (1:300 dilution). This product is for research use only. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NALP1 antibody can be stored at 4˚C, stable for one year. |
| Storage Buffer | PBS containing 0.02% sodium azide |
