Application
Western blot analysis of NALP4 in K562 cell lysate with NALP4 antibody at 1 μg/mL.| Product Name | NALP4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NALP4 Antibody: NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins and are thought to be involved in inflammation and reproduction. NALP4, also known as PYRIN-containing APAF1-like protein 4, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. In transfected 293 cells, NALP4 suppressed NF-κB induction by TNF-α and IL-1β, suggesting NALP4 operates at a point of convergence in these two signaling pathways. NALP4 also suppressed NF-κB induction resulting from overexpression of several adapter proteins and protein kinases involved in these pathways, including TRAF2, TRAF6, RIP, and IRAK2, as well as the IKK-alpha and IKK-beta, suggesting that NALP4 is critical in modulating NF-κB activity.1) Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104. |
| Immunogen | NALP4 antibody was raised against a 13 amino acid synthetic peptide near the center of human NALP4. The immunogen is located within amino acids 330 - 380 of NALP4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NALP4 antibody can be used for detection of NALP4 by Western blot at 1 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRP4 |
|---|---|
| Alternative Names | NALP4 Antibody: CT58 PAN2 RNH2 NALP4 PYPAF4 CLR19.5 NACHT LRR and PYD domains-containing protein 4 PAAD and NACHT-containing protein 2 CT58 |
Application
Western blot analysis of NALP4 in K562 cell lysate with NALP4 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
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| Application Notes | NALP4 antibody can be used for detection of NALP4 by Western blot at 1 μg/mL. |
| Form | Liquid |
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| Storage Instructions | NALP4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NALP4 Antibody is supplied in PBS containing 0.02% sodium azide. |
