Application
Immunofluorescence of NALP7 in human brain tissue with NALP7 antibody at 20 μg/mL.Application
Immunohistochemistry of NALP7 in human brain tissue with NALP7 antibody at 10 μg/mL.Application
Western blot analysis of NALP7 in K562 cell lysate with NALP7 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | NALP7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NALP7 Antibody: NOD proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins. NALP7, also known as PYPAF3, a member of the related PYRIN-containing APAF1-like proteins (PYPAFs/NALPs) is thought to play a crucial role in cell proliferation. NALP7 has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. It is expressed in numerous tissues including uterus and ovary, with low levels in heart and brain. NALP7 inhibits caspase-1-dependent interleukin-1β secretion and is a feedback regulator of interleukin-1β secretion. Defects in the NALP7 gene are known to cause the formation of a hydatidiform mole (HYDM) and reduce the growth of carcinoma cell lines.1) Tschopp J, Martinon F, and Burns K. NALPs: a novel protein family involved in inflammation. Nat. Rev. Mol. Cell Biol.2003; 4:95-104. |
| Immunogen | NALP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP7. The immunogen is located within amino acids 90 - 140 of NALP7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NALP7 antibody can be used for detection of NALP7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NLRP7 |
|---|---|
| Alternative Names | NALP7 Antibody: HYDM PAN7 NALP7 NOD12 PYPAF3 CLR19.4 NACHT LRR and PYD domains-containing protein 7 Nucleotide-binding oligomerization domain protein 12 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of NALP7 in human brain tissue with NALP7 antibody at 20 μg/mL.
Application
Immunohistochemistry of NALP7 in human brain tissue with NALP7 antibody at 10 μg/mL.
Application
Western blot analysis of NALP7 in K562 cell lysate with NALP7 antibody at 2 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | NALP7 antibody can be used for detection of NALP7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NALP7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NALP7 Antibody is supplied in PBS containing 0.02% sodium azide. |
