| Product Name | Nampt Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. |
| Immunogen | Synthetic peptide corresponding to aa 40-56 (R40EKKTENSKLRKVKYEE56) of human Nampt (Visfatin/PBEF). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHC |
| ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (frozen sections). Optimal conditions must be determined individually for each application. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | NAMPT |
|---|---|
| Alternative Names | Pre-B Cell Colony Enhancing Factor 1 PBEF1 Nicotinamide Phosphoribosyltransferase |
| Tissue Specificity | Recognizes human Nampt. Weakly cross-reacts with mouse Nampt. Detects a band of ~54kDa by Western blot. |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (frozen sections). Optimal conditions must be determined individually for each application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 6 months after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
