WB
Western Blot. Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate. All lanes: NAMPT antibody at 2.7µg/ml. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
Immunohistochemistry of paraffin-embedded human bladder cancer using Rabbit anti-NAMPT Polyclonal antibody at dilution of 1:100.ICC/IF
Immunofluorescent analysis of A549 cells using Rabbit anti-NAMPT Polyclonal antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-NAMPT Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Nicotinamide phosphoribosyltransferase protein (1-280AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | NAMPT |
|---|---|
| Gene Synonyms | VF PBEF PBEF1 VISFATIN 1110035O14Rik |
| Gene Full Name | nicotinamide phosphoribosyltransferase |
| Gene Summary | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011] |
| Alternative Names | 1110035O14Rik antibody AI314458 antibody AI480535 antibody DKFZP666B131 antibody EC 2.4.2.12 antibody MGC117256 antibody NAmPRTase antibody Nampt antibody NAMPT_HUMAN antibody Nicotinamide phosphoribosyltransferase antibody PBEF 1 antibody PBEF antibody PBEF1 antibody Pre B cell colony enhancing factor 1 antibody Pre B cell colony enhancing factor antibody Pre B cell enhancing factor antibody Pre-B cell-enhancing factor antibody Pre-B-cell colony-enhancing factor 1 antibody VF antibody Visfatin antibody |
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Cytoplasm,Nucleus,Secreted. |
WB
Western Blot. Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate. All lanes: NAMPT antibody at 2.7µg/ml. Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
Immunohistochemistry of paraffin-embedded human bladder cancer using Rabbit anti-NAMPT Polyclonal antibody at dilution of 1:100.
ICC/IF
Immunofluorescent analysis of A549 cells using Rabbit anti-NAMPT Polyclonal antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:20-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. |
