Western blot
Host: Rabbit Target Name: CHAD Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: NSUN6 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name:PBEF1 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%Western blot
1.25ug/mlPositive Control: Jurkat cell lysate| Product Name | Rabbit anti-PBEF1 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110035O14Rik, DKFZP666B131, MGC117256, NAMPT, PBEF, VF, PBEF1, VISFATIN |
| Immunogen | The immunogen for anti-PBEF1 antibody: synthetic peptide directed towards the C terminal of human PBEF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PBEF1 |
|---|---|
| Gene Synonyms | 1110035O14Rik DKFZP666B131 MGC117256 NAMPT PBEF VF PBEF1 VISFATIN |
| Gene Full Name | Nicotinamide phosphoribosyltransferase |
| Gene Summary | PBEF1 catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. |
| Alternative Names | 1110035O14Rik DKFZP666B131 MGC117256 NAMPT PBEF VF PBEF1 VISFATIN |
| Molecular Weight(MW) | 54kDa |
| Sequence | 491 amino acids |
Western blot
Host: Rabbit Target Name: CHAD Sample Tissue: Human Adult Placenta Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: NSUN6 Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name:PBEF1 Sample Tissue:JurkatLane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration:2.5ug/mL Peptide Concentration: 2.0ug/mL Lysate Quantity: 25ug/lane Gel Concentration: 12%
Western blot
1.25ug/mlPositive Control: Jurkat cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
