WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PBEF antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human bladder cancer was stained with anti-PBEF Rabbit antibody.IHC
Human breast carcinoma was stained with anti-PBEF Rabbit antibody| Product Name | PBEF Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:400-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NAMPT |
|---|---|
| Gene Synonyms | VF PBEF PBEF1 VISFATIN 1110035O14Rik |
| Gene Full Name | nicotinamide phosphoribosyltransferase |
| Gene Summary | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011] |
| Alternative Names | Nicotinamide phosphoribosyltransferase NAmPRTase Nampt Pre-B-cell colony-enhancing factor 1 Pre-B cell-enhancing factor Visfatin |
| Molecular Weight(MW) | 56 kDa |
| Cellular Localization | Nucleus . Cytoplasm . Secreted . Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PBEF antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human bladder cancer was stained with anti-PBEF Rabbit antibody.
IHC
Human breast carcinoma was stained with anti-PBEF Rabbit antibody| Application Notes | WB:1:2000-1:10000 IHC:1:400-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
