| Product Name | Nampt Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. |
| Immunogen | Recombinant mouse Nampt (visfatin/PBEF). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Gene Symbol | Nampt |
|---|---|
| Alternative Names | Pre-B Cell Colony Enhancing Factor 1 PBEF1 Nicotinamide Phosphoribosyltransferase |
| Tissue Specificity | Recognizes mouse Nampt. Weakly cross-reacts with human Nampt. Detects a band of ~54kDa by Western blot. |
| Application Notes | ELISA: (direct and indirect: 1:2,000-1:5,000). Western Blot: (1:2,000-1:5,000 using ECL suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 6 months after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
