Application
Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 μg/mL.Application
Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 μg/mL.Application
Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NANOG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NANOG Antibody: Expression of NANOG is required for the maintenance of pluripotency in epiblast and embryonic stem (ES) cells as well as for the ability to maintain ES self-renewal independently of LIF/Stat3. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.1) Chambers I, Colby D, Robertson M, et al. Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell2003; 113:643-55. |
| Immunogen | NANOG antibody was raised against a 19 amino acid synthetic peptide near the center of human NANOG. The immunogen is located within amino acids 130 - 180 of NANOG. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NANOG |
|---|---|
| Alternative Names | NANOG Antibody: Homeobox protein NANOG Homeobox transcription factor Nanog hNanog |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 μg/mL.
Application
Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 μg/mL.
Application
Western blot analysis of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | NANOG antibody can be used for detection of NANOG by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NANOG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NANOG Antibody is supplied in PBS containing 0.02% sodium azide. |
