Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Product Name | NANOG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.1) Piestun, D., et al., (2006) Biochem. Biophys. Res. Commun. 343 (1), 279-285. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NANOG. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NANOG antibody can be used for detection of NANOG by ELISA at 1:1562500. NANOG antibody can be used for detection of NANOG by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NANOG |
|---|---|
| Alternative Names | NANOG |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.| Application Notes | NANOG antibody can be used for detection of NANOG by ELISA at 1:1562500. NANOG antibody can be used for detection of NANOG by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NANOG antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
