Application
Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using Nano g Antibody.| Product Name | Nanog Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation1) Do HJ, et al. Biochem Biophys Res Commun. 2007 Feb 16;353 (3): 770-5. Boyer LA, et al. Cell. 2005 Sep 23;122 (6): 947-56. Freberg CT, et al. Mol Biol Cell. 2007 May;18 (5): 1543-53. Chambers I, et al. Cell. 2003 May 30;113 (5): 643-55. |
| Immunogen | Nanog antibody was raised against a peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NANOG |
|---|---|
| Alternative Names | FLK1 VEGFR2 Homeobox transcription factor Nanog |
| Molecular Weight(MW) | 42 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Nanog protein. |
| Entrez Gene | 79923 |
|---|---|
| Protein Accession | NP_079141.2 |
Application
Western blot analysis of lysed extracts from human umbilical cord mesenchymal stem cell using Nano g Antibody.| Application Notes | Western Blot: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
