Application
Immunohistochemistry of Nanos1 in human brain tissue with Nanos1 antibody at 2.5 μg/mLApplication
Immunofluorescence of nanos1 in human brain tissue with nanos1 antibody at 20 μg/mL.Application
Western blot analysis of Nanos1 in SK-N-SH cell lysate with Nanos1 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.| Product Name | Nanos1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Nanos1 Antibody: Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.1) Jaruleska J, Kotecki M, Kusz K, et al. Conservation of a Pumilio-Nanos complex from Drosophila germ plasm to human germ cells. Dev. Genes Evol.2003; 213:120-6. |
| Immunogen | Nanos1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Nanos1. The immunogen is located within the first 50 amino acids of Nanos1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NANOS1 |
|---|---|
| Alternative Names | Nanos1 Antibody: NOS1 SPGF12 NOS1 Nanos homolog 1 NOS-1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of Nanos1 in human brain tissue with Nanos1 antibody at 2.5 μg/mL
Application
Immunofluorescence of nanos1 in human brain tissue with nanos1 antibody at 20 μg/mL.
Application
Western blot analysis of Nanos1 in SK-N-SH cell lysate with Nanos1 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Nanos1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Nanos1 Antibody is supplied in PBS containing 0.02% sodium azide. |
