Application
Immunohistochemistry of Nanos2 in human brain tissue cells with Nanos2 antibody at 2.5 μg/mL.Application
Immunofluorescence of Nanos2 in Human Testis cells with Nanos2 antibody at 20 μg/mL.Application
Western blot analysis of Nanos2 in rat brain tissue lysate with Nanos2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Nanos2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Nanos2 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos2 antibody will not cross-react with either Nanos 1 or Nanos3.1) Lehmann R and Nusslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the drosophila embryo. Development1991; 112:679-91. |
| Immunogen | Nanos2 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of the human Nanos2. The immunogen is located within the first 50 amino acids of Nanos2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Nanos2 antibody can be used for detection of Nanos2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NANOS2 |
|---|---|
| Alternative Names | Nanos2 Antibody: NOS2 NOS2 Nanos homolog 2 NOS-2 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of Nanos2 in human brain tissue cells with Nanos2 antibody at 2.5 μg/mL.
Application
Immunofluorescence of Nanos2 in Human Testis cells with Nanos2 antibody at 20 μg/mL.
Application
Western blot analysis of Nanos2 in rat brain tissue lysate with Nanos2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | Nanos2 antibody can be used for detection of Nanos2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Nanos2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Nanos2 Antibody is supplied in PBS containing 0.02% sodium azide. |
