Application
Immunofluorescence of Nanos3 in Human Brain tissue with Nanos3 antibody at 20 μg/mL.Application
Immunohistochemistry of Nanos3 in human brain tissue with Nanos3 antibody at 2.5 μg/mL.Application
Western blot analysis of Nanos3 in human brain tissue lysate with Nanos3 antibody at 2 μg/mL.| Product Name | Nanos3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Nanos3 Antibody: Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects.1) Lehmann R and Nusslein-Volhard C. The maternal gene nanos has a central role in posterior pattern formation of the drosophila embryo. Development 1991; 112:679-91. |
| Immunogen | Nanos3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3. The immunogen is located within the last 50 amino acids of Nanos3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Nanos3 antibody can be used for detection of Nanos3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NANOS3 |
|---|---|
| Alternative Names | Nanos3 Antibody: NOS3 NANOS1L NOS3 Nanos homolog 3 NOS-3 |
| Molecular Weight(MW) | Predicted: 19 kDa Observed: 20 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2. |
Application
Immunofluorescence of Nanos3 in Human Brain tissue with Nanos3 antibody at 20 μg/mL.
Application
Immunohistochemistry of Nanos3 in human brain tissue with Nanos3 antibody at 2.5 μg/mL.
Application
Western blot analysis of Nanos3 in human brain tissue lysate with Nanos3 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | Nanos3 antibody can be used for detection of Nanos3 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Nanos3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Nanos3 Antibody is supplied in PBS containing 0.02% sodium azide. |
