Application
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human NAP-2 (XP-5244Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2.| Product Name | NAP-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNAP-2 (human Neutrophil Activating Protein-2). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] oof the biological activity of hNAP-2 (50.00 ng/mL), a concentration of 1.75 - 3.0 μg/mL of this antibody is required. ELISA: To detect hNAP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2. Sandwich: To detect hNAP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NAP-2 (XP-5244Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPBP |
|---|---|
| Alternative Names | PBP TC1 TC2 TGB LDGF MDGF TGB1 B-TG1 CTAP3 CXCL7 NAP-2 SCYB7 THBGB LA-PF4 THBGB1 Beta-TG CTAPIII CTAP-III Platelet basic protein C-X-C motif chemokine 7 PBP |
Application
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hNAP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human NAP-2 (XP-5244Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] oof the biological activity of hNAP-2 (50.00 ng/mL), a concentration of 1.75 - 3.0 μg/mL of this antibody is required. ELISA: To detect hNAP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hNAP-2. Sandwich: To detect hNAP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human NAP-2 (XP-5244Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NAP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
