| Product Name | NAP-2 Antibody [G6_5C7.3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells. |
| Immunogen | The monoclonal antibody was produced in BALB/c mice using recombinant human NAP-2 as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human NAP-2 when used with our biotinylated antigen affinity purified anti-human NAP-2 (XP-5244Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPBP |
|---|---|
| Alternative Names | PPBP CXCL7 CTAP3 SCYB7 TGB1 THBGB1 Platelet basic protein C-X-C motif chemokine 7 PBP TC1 TC2 TGB LDGF MDGF B-TG1 NAP-2 THBGB LA-PF4 Beta-TG CTAPIII CTAP-III |
| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human NAP-2 when used with our biotinylated antigen affinity purified anti-human NAP-2 (XP-5244Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNAP-2 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | NAP-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
