Application
Immunofluorescence of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 20 μg/mL.Application
Immunohistochemistry of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 5 μg/mL.Application
Western blot analysis of NAP1L5 in EL4 cell lysate with NAP1L5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | NAP1L5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The nucleosome assembly protein 1-like 5 (NAP1L5) protein, also known as DRLM, was initially identified as a possible tumor suppressor gene in hepatocellular carcinoma (1). The NAP1L5 gene is located within the intron of Herc3, and unlike Herc3, is imprinted and highly expressed in regions of the adult mouse brain (2).1) Harada H, Nagai H, Ezura Y, et al. Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein. Gene 2002; 296:171-7. |
| Immunogen | NAP1L5 antibody was raised against an 18 amino acid peptide near the center of human NAP1L5. The immunogen is located within amino acids 40 - 90 of NAP1L5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NAP1L5 antibody can be used for detection of NAP1L5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NAP1L5 |
|---|---|
| Alternative Names | Nucleosome assembly protein 1-like 5 DRLM down-regulated in liver malignancy |
| Molecular Weight(MW) | Predicted: 20 kDa (17kDa in mouse) Observed: 15 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | NAP1L5 antibody is human, mouse and rat reactive. |
Application
Immunofluorescence of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 20 μg/mL.
Application
Immunohistochemistry of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 5 μg/mL.
Application
Western blot analysis of NAP1L5 in EL4 cell lysate with NAP1L5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | NAP1L5 antibody can be used for detection of NAP1L5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NAP1L5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NAP1L5 antibody is supplied in PBS containing 0.02% sodium azide. |
