Application
Immunohistochemistry of NAT11 in mouse thymus tissue with NAT11 antibody at 5 μg/mL.Application
Immunofluorescence of NAT11 in mouse thymus tissue with NAT11 antibody at 20 μg/mL.Application
Western blot analysis of NAT11 in human thymus tissue lysate with NAT11 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NAT11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NAT11 Antibody: N-terminal acetylation is one of the most common protein modifications in eukaryotes, occurring on approximately 57% and 84% on yeast and human proteins respectively. There are several N-terminal acetylating enzyme complexes (NatA - NatE). Unlike the other complexes, NatD is composed of a single protein, NAT11, and has recently been described to acetylate the Serine N-termini of histones H2A and H4 in yeast. The role these modifications play is unknown; yeast that do not express NAT11 grow at normal rates and have no observable phenotypes. The role of the human homolog is likewise unknown.1) Arnesen T, Van Damme P, Polevoda B, et al. Proteomics analyses reveal the evolutionary conservation and divergence of N-terminal acetyltransferases from yeast and humans. Proc. Natl. Acad. Sci. USA2009; 106:8157-62. |
| Immunogen | NAT11 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human NAT11. The immunogen is located within the last 50 amino acids of NAT11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NAT11 antibody can be used for detection of NAT11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NAA40 |
|---|---|
| Alternative Names | NAT11 Antibody: NAT11 PATT1 NAT11 N-alpha-acetyltransferase 40 N-acetyltransferase 11 |
Application
Immunohistochemistry of NAT11 in mouse thymus tissue with NAT11 antibody at 5 μg/mL.
Application
Immunofluorescence of NAT11 in mouse thymus tissue with NAT11 antibody at 20 μg/mL.
Application
Western blot analysis of NAT11 in human thymus tissue lysate with NAT11 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1314 - Human Thymus Tissue Lysate |
|---|---|
| Application Notes | NAT11 antibody can be used for detection of NAT11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NAT11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NAT11 Antibody is supplied in PBS containing 0.02% sodium azide. |
