Application
Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.Application
Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.Application
Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | NCLN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.1) Haffner C, Frauli M, Topp S, et al. Nicalin and its binding partner Nomo are novel Nodal signaling antagonists. EMBO J. 2004; 23:3041-50. |
| Immunogen | NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN. The immunogen is located within amino acids 390 - 440 of NCLN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCLN |
|---|---|
| Alternative Names | NCLN Antibody: NET59 Nicalin Nicastrin-like protein |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.
Application
Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.
Application
Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | NCLN antibody can be used for detection of NCLN by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | NCLN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NCLN Antibody is supplied in PBS containing 0.02% sodium azide. |
