NCOR1 mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p.1) Horlein, A.J., et al., (1995) Nature 377 (6548), 397-404.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCOR1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NCOR1 antibody can be used for detection of NCOR1 by ELISA at 1:62500. NCOR1 antibody can be used for detection of NCOR1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Small Intestine at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Intestine.
Positive Control
1) Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate
Application Notes
NCOR1 antibody can be used for detection of NCOR1 by ELISA at 1:62500. NCOR1 antibody can be used for detection of NCOR1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NCOR1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.