Application
Antibody used in WB on Human HeLa at 2.5 ug/ml.| Product Name | NCOR2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCOR2, SMRT, TRAC, CTG26, SMRTE, TRAC1, N-CoR2, TNRC14, TRAC-1, SMAP270, SMRTE-tau |
| Product description | NCOR2 forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid (TR) and the retinoid acid receptors (RAR) in the absence of ligand, and may stabilize their interaction with TFIIB. NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.1) Keeton, E.K. (2005) Mol. Endocrinol. 19 (6), 1543-1554. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCOR2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NCOR2 antibody can be used for detection of NCOR2 by ELISA at 1:62500. NCOR2 antibody can be used for detection of NCOR2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NCOR2 |
|---|---|
| Alternative Names | NCOR2 SMRT TRAC CTG26 SMRTE TRAC1 N-CoR2 TNRC14 TRAC-1 SMAP270 SMRTE-tau |
| Molecular Weight(MW) | 275 kDa |
Application
Antibody used in WB on Human HeLa at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | NCOR2 antibody can be used for detection of NCOR2 by ELISA at 1:62500. NCOR2 antibody can be used for detection of NCOR2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NCOR2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
