Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ND2 Antibody in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ND2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NADH-ubiquinone oxidoreductase chain 2, NADH dehydrogenase subunit 2, MT-ND2, MTND2, NADH2, ND2 |
| Product description | ND2 is core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. This protein complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone1) Saitoh,K., et.al., J. Mol. Evol. 63 (6), 826-841 (2006) |
| Immunogen | This ND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human ND2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MT-ND2 |
|---|---|
| Alternative Names | NADH-ubiquinone oxidoreductase chain 2 NADH dehydrogenase subunit 2 MT-ND2 MTND2 NADH2 ND2 |
| Molecular Weight(MW) | 39 kDa |
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ND2 Antibody in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
