Application
Flow cytometric analysis of CEM cells using ND5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ND5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NADH-ubiquinone oxidoreductase chain 5, NADH dehydrogenase subunit 5, MT-ND5, MTND5, NADH5, ND5 |
| Product description | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Immunogen | This ND5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-570 amino acids from the C-terminal region of human ND5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MT-ND5 |
|---|---|
| Alternative Names | NADH-ubiquinone oxidoreductase chain 5 NADH dehydrogenase subunit 5 MT-ND5 MTND5 NADH5 ND5 |
| Molecular Weight(MW) | 67 kDa |
Application
Flow cytometric analysis of CEM cells using ND5 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ND5 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
