Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | NDE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDE1, FLJ20101, HOM-TES-87, NUDE, NUDE1, NDE, LIS4 |
| Product description | NDE1 is required for centrosome duplication and formation and function of the mitotic spindle. It is essential for the development of the cerebral cortex. NDE1 may regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex1) Burdick, K.E., (er) Hum. Mol. Genet. (2008) In press. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NDE1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NDE1 antibody can be used for detection of NDE1 by ELISA at 1:62500. NDE1 antibody can be used for detection of NDE1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDE1 |
|---|---|
| Alternative Names | NDE1 FLJ20101 HOM-TES-87 NUDE NUDE1 NDE LIS4 |
| Molecular Weight(MW) | 38 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NDE1 antibody can be used for detection of NDE1 by ELISA at 1:62500. NDE1 antibody can be used for detection of NDE1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NDE1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
