Application
Immunofluorescence of NDFIP1 in human lung tissue with NDFIP1 antibody at 20 μg/ml.Application
Immunohistochemistry of NDFIP1 in human lung tissue with NDFIP1 antibody at 5 μg/ml.Application
Western blot analysis of NDFIP1 in PC-3 cell lysate with NDFIP1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | NDFIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDFIP1 Antibody: N4WBP5, N4WBP5, PSEC0192, PSEC0223, NEDD4 family-interacting protein 1, Breast cancer-associated protein SGA-1M |
| Product description | NDFIP1 Antibody: The NEDD4 family-interacting protein 1 (NDFIP1) belongs to a small group of evolutionarily conserved proteins with three transmembrane domains and is an integral Golgi membrane protein. It is a potential target for ubiquitination by the Nedd4 family of proteins. NDFIP1 is strongly expressed in surviving neurons following acute cortical brain injury, and overexpression in cultured cortical neurons increased survival following growth factor starvation, suggesting that NDFIP1 may play a role in neuronal survival. NDFIP1 and the related protein NDFIP2 are thought to interact with and regulate multiple components of the EGF and PTEN/Akt signaling pathways. Recent studies suggest that NDFIP1 may also play a role in Th17 differentiation by limiting the production of proinflammatory cytokines.1) Harvey KF, Shearwin-Whyatt LM, Fotia A, et al. N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. J. Biol. Chem. 2002; 277:9307-17. |
| Immunogen | NDFIP1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NDFIP1. The immunogen is located within the first 50 amino acids of NDFIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NDFIP1 antibody can be used for detection of NDFIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDFIP1 |
|---|---|
| Alternative Names | NDFIP1 Antibody: N4WBP5 N4WBP5 PSEC0192 PSEC0223 NEDD4 family-interacting protein 1 Breast cancer-associated protein SGA-1M |
| Molecular Weight(MW) | 24 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | NDFIP1 antibody is predicted to not cross-react with NDFIP2. |
Application
Immunofluorescence of NDFIP1 in human lung tissue with NDFIP1 antibody at 20 μg/ml.
Application
Immunohistochemistry of NDFIP1 in human lung tissue with NDFIP1 antibody at 5 μg/ml.
Application
Western blot analysis of NDFIP1 in PC-3 cell lysate with NDFIP1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | NDFIP1 antibody can be used for detection of NDFIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NDFIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NDFIP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
