Application
Immunofluorescence of NDST2 in Daudi cells with NDST2 antibody at 20 μg/ml.Application
Western blot analysis of NDST2 in A-20 cell lysate with NDST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | NDST2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDST2 Antibody: NST2, HSST2, N-heparan sulfate sulfotransferase 2, NDST-2 |
| Product description | The N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2) protein, also known as N-heparan sulfate sulfotransferase 2, and the highly related protein NDST1 have key roles in the sulfation of heparan sulfate proteoglycans, molecules that are present of cell surfaces and the extracellular matrix (1,2). NDST1 and NDST2 are dispensable for mesodermal differentiation into osteoblasts but necessary for induction of adipocytes and neural cells (3).1) Humphries DE, Lanciotti J, and Karlinsky JB. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2. Biochem. J. 1998; 332:303-7. |
| Immunogen | NDST2 antibody was raised against a 16 amino acid peptide near the amino terminus of human NDST2. The immunogen is located within amino acids 20 - 70 of NDST2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| NDST2 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDST2 |
|---|---|
| Alternative Names | NDST2 Antibody: NST2 HSST2 N-heparan sulfate sulfotransferase 2 NDST-2 |
| Molecular Weight(MW) | Predicted: 97 kDa Observed: 101 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | NDST2 antibody is human, mouse and rat reactive. At least two isoforms of NDST2 are known to exist; this antibody will only detect the larger isoform. NDST2 antibody is predicted not to cross-react with other members of the NDST protein family. |
Application
Immunofluorescence of NDST2 in Daudi cells with NDST2 antibody at 20 μg/ml.
Application
Western blot analysis of NDST2 in A-20 cell lysate with NDST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | NDST2 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NDST2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NDST2 antibody is supplied in PBS containing 0.02% sodium azide. |
