Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | NDUFB5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NDUFB5, CI-SGDH, DKFZp686N02262, FLJ30597, MGC111204, MGC12314, SGDH, CISGDH |
| Product description | NDUFB5 is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.1) Ton, C., (1997) Biochem. Biophys. Res. Commun. 241 (2), 589-594. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NDUFB5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NDUFB5 antibody can be used for detection of NDUFB5 by ELISA at 1:62500. NDUFB5 antibody can be used for detection of NDUFB5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFB5 |
|---|---|
| Alternative Names | NDUFB5 CI-SGDH DKFZp686N02262 FLJ30597 MGC111204 MGC12314 SGDH CISGDH |
| Molecular Weight(MW) | 17 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NDUFB5 antibody can be used for detection of NDUFB5 by ELISA at 1:62500. NDUFB5 antibody can be used for detection of NDUFB5 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NDUFB5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
