Application
Western blot analysis of NDUFS4 using rabbit polyclonal NDUFS4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFS4 gene.Application
Flow cytometric analysis of ATDC5 cells using NDUFS4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of NDUFS4 Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | NDUFS4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial, Complex I-18 kDa, CI-18 kDa, Complex I-AQDQ, CI-AQDQ, NADH-ubiquinone oxidoreductase 18 kDa subunit, NDUFS4 |
| Product description | NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase(Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.1) Panelli,D., et.al., Biochimie 90 (10), 1452-1460 (2008) |
| Immunogen | This NDUFS4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the C-terminal region of human NDUFS4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFS4 |
|---|---|
| Alternative Names | NADH dehydrogenase [ubiquinone] iron-sulfur protein 4 mitochondrial Complex I-18 kDa CI-18 kDa Complex I-AQDQ CI-AQDQ NADH-ubiquinone oxidoreductase 18 kDa subunit NDUFS4 |
| Molecular Weight(MW) | 20 kDa |
Application
Western blot analysis of NDUFS4 using rabbit polyclonal NDUFS4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NDUFS4 gene.
Application
Flow cytometric analysis of ATDC5 cells using NDUFS4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with NDUFS4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of NDUFS4 Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
