Application
NDUFS8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Product Name | NDUFS8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, Complex I-23kD, CI-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, NDUFS8 |
| Product description | This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)Bourges, I., et al. Biochem. J. 383 (PT 3), 491-499 (2004)Procaccio, V., et al. Neurology 62(10):1899-1901(2004) Ugalde, C., et al. Hum. Mol. Genet. 13(6):659-667(2004) Murray, J., et al. J. Biol. Chem. 278(39):37223-37230(2003) |
| Immunogen | This NDUFS8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the Central region of human NDUFS8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NDUFS8 |
|---|---|
| Alternative Names | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8 mitochondrial Complex I-23kD CI-23kD NADH-ubiquinone oxidoreductase 23 kDa subunit TYKY subunit NDUFS8 |
| Molecular Weight(MW) | 24 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
NDUFS8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
