Variation in NEDD9 is associated wih susceptibility to late-onset Alzheimer and Parkinson diseases. Changes in expression of the scaffold protein HEF1/CAS-L/NEDD9 were found to be a potent prometastatic stimulus in melanoma and other cancers.1) Li, Y., (2008) Hum. Mol. Genet. 17 (5), 759-767.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NEDD9.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NEDD9 antibody can be used for detection of NEDD9 by ELISA at 1:62500. NEDD9 antibody can be used for detection of NEDD9 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human 721_B cells at 5.0 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
NEDD9 antibody can be used for detection of NEDD9 by ELISA at 1:62500. NEDD9 antibody can be used for detection of NEDD9 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NEDD9 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt