Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).Application
NEDD9 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | NEDD9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B-and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.1) Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010) |
| Immunogen | This NEDD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-363 amino acids from the Central region of human NEDD9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEDD9 |
|---|---|
| Alternative Names | Enhancer of filamentation 1 hEF1 CRK-associated substrate-related protein CAS-L CasL Cas scaffolding protein family member 2 Neural precursor cell expressed developmentally down-regulated protein 9 NEDD-9 Renal carcinoma antigen NY-REN-12 p105 En |
| Molecular Weight(MW) | 93 kDa |
Application
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Application
NEDD9 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
