Application
Western blot analysis of NEK3 using rabbit polyclonal NEK3 Antibody (K346) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NEK3 gene.Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | NEK3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, NEK3 |
| Product description | In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. NEK3 is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm.1) Schultz, S.J., et al., Cell Growth Differ. 5(6):625-635 (1994). |
| Immunogen | This NEK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the Central region of human NEK3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEK3 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase Nek3 HSPK 36 Never in mitosis A-related kinase 3 NimA-related protein kinase 3 NEK3 |
| Molecular Weight(MW) | 58 kDa |
Application
Western blot analysis of NEK3 using rabbit polyclonal NEK3 Antibody (K346) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NEK3 gene.
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
