Application
Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 μg/mL.Application
Immunofluorescence of NENF in rat kidney tissue with NENF antibody at 20 μg/mL.Application
Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NENF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NENF Antibody: CIR2, SPUF, SCIRP10, CIR2, Neudesin, Cell immortalization-related protein 2, SPUF protein |
| Product description | NENF Antibody: NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.1) Kimura I, Yoshioka M, Konishi M, et al. Neudesin, a novel secreted protein with a unique primary structure and neurotrophic activity. J. Neurosci. Res.2005; 79:287-94. |
| Immunogen | NENF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF. The immunogen is located within the last 50 amino acids of NENF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NENF |
|---|---|
| Alternative Names | NENF Antibody: CIR2 SPUF SCIRP10 CIR2 Neudesin Cell immortalization-related protein 2 SPUF protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Immunohistochemistry of NENF in rat kidney tissue with NENF antibody at 10 μg/mL.
Application
Immunofluorescence of NENF in rat kidney tissue with NENF antibody at 20 μg/mL.
Application
Western blot analysis of NENF in human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | NENF antibody can be used for detection of NENF by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NENF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NENF Antibody is supplied in PBS containing 0.02% sodium azide. |
