Application
Immunohistochemistry of NETO1 in rat lung tissue with NETO1 antibody at 2.5 μg/mL.Application
Immunofluorescence of NETO1 in rat lung tissue with NETO1 antibody at 20 μg/mL.Application
Western blot analysis of NETO1 in human brain tissue lysate with NETO1 antibody at 0.25 μg/mL.| Product Name | NETO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NETO1 Antibody: BCTL1, BTCL1, Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 |
| Product description | NETO1 Antibody: Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR), it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.1) Michishita M, Ikeda T, Nakashiba T, et al. A novel gene, Btcl1, encoding CUB and LDLa domains is expressed in restricted areas of mouse brain. Biochem. Biophys. Res. Commun. 2003; 306:680-6. |
| Immunogen | NETO1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NETO1. The immunogen is located within amino acids 350 - 400 of NETO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NETO1 |
|---|---|
| Alternative Names | NETO1 Antibody: BCTL1 BTCL1 Neuropilin and tolloid-like protein 1 Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 |
| Molecular Weight(MW) | Predicted: 60 kDa Observed: 60 kDa |
| Tissue Specificity | NETO1 antibody is predicted to not cross-react with other NETO protein family members. |
Application
Immunohistochemistry of NETO1 in rat lung tissue with NETO1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of NETO1 in rat lung tissue with NETO1 antibody at 20 μg/mL.
Application
Western blot analysis of NETO1 in human brain tissue lysate with NETO1 antibody at 0.25 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NETO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NETO1 Antibody is supplied in PBS containing 0.02% sodium azide. |
