Application
Immunohistochemistry of NEUCRIN in mouse brain tissue with NEUCRIN antibody at 5 μg/mL.Application
Immunofluorescence of NEUCRIN in mouse brain tissue with NEUCRIN antibody at 20 μg/mL.Application
Western blot analysis of Neucrin in rat cerebellum tissue lysate with Neucrin antibody at 1 μg/mL.| Product Name | NEUCRIN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEUCRIN Antibody: UNQ3119, neucrin, AGPA3119, C1orf187, PSEC0258, UNQ3119/PRO10268, Draxin, Dorsal inhibitory axon guidance protein |
| Product description | NEUCRIN Antibody: Neucrin, also known as Draxin, is a repulsive guidance protein for the spinal cord and forebrain commissures. It is thought to act as an antagonist of canonical Wnt signaling by inhibiting the stabilization of cytosolic beta-catenin. Ectopically expressed neucrin inhibited growth or caused misrouting of chick spinal cord commissural axons in vivo while Neucrin-null mice showed defasciculation of spinal cord commissural axons and an absence of all forebrain commisures. Other experiments in mice have shown that olfactory bulb axonal outgrowth is inhibited by Neucrin, suggesting that Neucrin functions as an inhibitory guidance cue for olfactory bulb axons.1) Islam SM, Shinmyo Y, Okafuji T, et al. Draxin, a repulsive guidance protein for spinal cord and forebrain commissures. Science 2009; 323:388-93. |
| Immunogen | Neucrin antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Neucrin. The immunogen is located within the last 50 amino acids of NEUCRIN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Neucrin antibody can be used for detection of Neucrin by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C1orf187 |
|---|---|
| Alternative Names | NEUCRIN Antibody: UNQ3119 neucrin AGPA3119 C1orf187 PSEC0258 UNQ3119/PRO10268 Draxin Dorsal inhibitory axon guidance protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%) |
| Tissue Specificity | At least three isoforms of Neucrin are known to exist; this antibody will detect the two shorter isoforms. Neucrin antibody is predicted to not cross-react with RILP. |
Application
Immunohistochemistry of NEUCRIN in mouse brain tissue with NEUCRIN antibody at 5 μg/mL.
Application
Immunofluorescence of NEUCRIN in mouse brain tissue with NEUCRIN antibody at 20 μg/mL.
Application
Western blot analysis of Neucrin in rat cerebellum tissue lysate with Neucrin antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1473 - Rat Cerebellum Tissue Lysate |
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| Application Notes | Neucrin antibody can be used for detection of Neucrin by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NEUCRIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NEUCRIN Antibody is supplied in PBS containing 0.02% sodium azide. |
