Application
Immunohistochemistry of Neuritin in mouse heart tissue with Neuritin antibody at 5 μg/mL.Application
Immunohistochemistry of Neuritin in human brain tissue with Neuritin antibody at 2.5 μg/ml.Application
Western blot analysis of neuritin in Daudi cell lysate with neuritin antibody at (A) 5 and (B) 10 μg/mL.Application
Immunofluorescence of Neuritin in Mouse Heart cells with Neuritin antibody at 20 μg/mL.| Product Name | Neuritin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuritin Antibody: NRN, dJ380B8.2, NRN, Neuritin |
| Product description | Neuritin Antibody: As the nervous system of a complex organism develops, it establishes functional networks through the growth and retraction of synaptic connections from growing axons and dendrites. This synaptic remodeling involves neuro-transmitter signaling, activation of neurotrophin receptors and alterations in gene expression. One such gene whose expression is increased by neural activity is neuritin, a GPI-anchored protein that is expressed in postmitotic differentiating neurons of the developing nervous system. Its expression is also induced by the neurotrophins BDNF and NT-3. Purified recombinant neuritin promotes neurite outgrowth and arborization in primary embryonic neuronal cultures, suggesting that neuritin may play a role as a downstream effector of activity-induced neurite outgrowth. More recent experiments have shown that neuritin is required for the androgen-induced axonal elongation in motor neurons and is upregulated following spinal cord injury, suggesting that neuritin may also play a role in survival and axonal regeneration.1) Li Z and Sheng M. Some assembly required: the development of neuronal synapses. Nat. Rev. Mol. Cell Biol. 2003; 4:833-41. |
| Immunogen | Neuritin antibody was raised against a 20 amino acid synthetic peptide from near the center of human neuritin. The immunogen is located within amino acids 70 - 120 of Neuritin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Neuritin antibody can be used for detection of Neuritin by Western blot at 5 and 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NRN1 |
|---|---|
| Alternative Names | Neuritin Antibody: NRN dJ380B8.2 NRN Neuritin |
| Molecular Weight(MW) | Predicted: 16 kDa Observed: 15 kDa |
| Sequence Similarities | 2) Cat. No. 1401 - Mouse Heart Tissue Lysate |
Application
Immunohistochemistry of Neuritin in mouse heart tissue with Neuritin antibody at 5 μg/mL.
Application
Immunohistochemistry of Neuritin in human brain tissue with Neuritin antibody at 2.5 μg/ml.
Application
Western blot analysis of neuritin in Daudi cell lysate with neuritin antibody at (A) 5 and (B) 10 μg/mL.
Application
Immunofluorescence of Neuritin in Mouse Heart cells with Neuritin antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | Neuritin antibody can be used for detection of Neuritin by Western blot at 5 and 10 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Neuritin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Neuritin Antibody is supplied in PBS containing 0.02% sodium azide. |
