Application
Immunohistochemistry of Neurotrypsin in human brain tissue with Neurotrypsin antibody at 2.5 μg/mL.Application
Immunofluorescence of Neurotrypsin in Human Brain cells with Neurotrypsin antibody at 20 μg/mL.Application
Western blot analysis of neurotrypsin in 3T3 cell lysate with neurotrypsin antibody at (A) 0.25 and (B) 0.5 μg/mL.| Product Name | Neurotrypsin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neurotrypsin Antibody: MRT1, BSSP3, BSSP-3, Neurotrypsin, Leydin |
| Product description | Neurotrypsin Antibody: Neurotrypsin is a central nervous system-expressed serine protease whose truncation or absence causes nonsyndromic mental retardation. It is most prominently expressed in structures that are involved in the processing and storage of learned behavior and memory, such as the cerebral cortex, the hippocampus, and amygdala. Evidence suggests that neurotrypsin has multiple functions, including axonal outgrowth, maintaining neuronal plasticity, and arranging the perineuronal environment, partly in coordination with other proteases including tissue plasminogen activator. At least two isoforms of neurotrypsin are known to exist.1) Gschwend TP, Krueger SR, Kozlov SV, et al. Neurotrypsin, a novel multidomain serine protease expressed in the nervous system. Mol. Cell. Neurosci.1997; 9:207-19. |
| Immunogen | Neurotrypsin antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human neurotrypsin. The immunogen is located within amino acids 110 - 160 of Neurotrypsin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Neurotrypsin antibody can be used for detection of neurotrypsin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRSS12 |
|---|---|
| Alternative Names | Neurotrypsin Antibody: MRT1 BSSP3 BSSP-3 Neurotrypsin Leydin |
| Molecular Weight(MW) | Predicted: 96 kDa Observed: 96 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of Neurotrypsin in human brain tissue with Neurotrypsin antibody at 2.5 μg/mL.
Application
Immunofluorescence of Neurotrypsin in Human Brain cells with Neurotrypsin antibody at 20 μg/mL.
Application
Western blot analysis of neurotrypsin in 3T3 cell lysate with neurotrypsin antibody at (A) 0.25 and (B) 0.5 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | Neurotrypsin antibody can be used for detection of neurotrypsin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Neurotrypsin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Neurotrypsin Antibody is supplied in PBS containing 0.02% sodium azide. |
