Application
Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | Neutrophil Cytosol Factor 1 (phospho Ser304) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCF1, NCF-47K, P47 phox |
| Product description | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).1) Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990). |
| Immunogen | Neutrophil Cytosol Factor 1 (phospho Ser304) antibody was raised against a peptide sequence around phosphorylation site of Serine 304 (R-S-S (p)-I-R) derived from Human Neutrophil Cytosol Factor 1. |
| Modification | p-Ser304 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCF1 |
|---|---|
| Alternative Names | NCF1 NCF-47K P47 phox |
| Molecular Weight(MW) | 44 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 304. |
| Entrez Gene | 653361 |
|---|---|
| Protein Accession | NP_000256.4 |
Application
Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Neutrophil Cytosol Factor 1 (Phospho-Ser304) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
