Application
Western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Neutrophil elastase antibody detected the Neutrophil elastase protein (arrow).| Product Name | Neutrophil elastase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, HLE, Medullasin, PMN elastase, ELANE, ELA2 |
| Product description | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.1) Kallquist, L., et al. Exp. Cell Res. 316(19):3182-3196(2010) |
| Immunogen | This Neutrophil elastase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-39 amino acids from the N-terminal region of human Neutrophil elastase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ELANE |
|---|---|
| Alternative Names | Neutrophil elastase Bone marrow serine protease Elastase-2 Human leukocyte elastase HLE Medullasin PMN elastase ELANE ELA2 |
| Molecular Weight(MW) | 29 kDa |
Application
Western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Neutrophil elastase antibody detected the Neutrophil elastase protein (arrow).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
