Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.Application
Antibody used in WB on HT-29 at: 1:1000 (Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl ).| Product Name | NFAT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFAT5, CAG-8, CAG80, OREBP, nfatz, NFATL1, TonEBP, AI225870, mKIAA0827, B130038B15Rik |
| Product description | Nfat5 contains 1 RHD (Rel-like) domain, and plays a role in the inducible expression of genes. Nfat5 regulates hypertonicity-induced cellular accumulation of osmolytes. Nfat5 might be capable of forming stable dimers with DNA elements1) Pan, S., et al., (2000) Biochem. Biophys. Res. Commun. 272 (3), 765-776. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse NFAT5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFAT5 antibody can be used for detection of NFAT5 by ELISA at 1:62500. NFAT5 antibody can be used for detection of NFAT5 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFAT5 |
|---|---|
| Alternative Names | NFAT5 CAG-8 CAG80 OREBP nfatz NFATL1 TonEBP AI225870 mKIAA0827 B130038B15Rik |
| Molecular Weight(MW) | 74 kDa |
Application
Antibody used in WB on Mouse SP2/0 at 0.2-1 ug/ml.
Application
Antibody used in WB on HT-29 at: 1:1000 (Lane 1: 20ug HT 29 lysate blocked with 5% FBS Lane 2: 20ug HT 29 lysate blocked with no FBS Lane 3: 20ug HT-29 lyaste blocked with no PBS + 100 mM NaCl ).| Positive Control | 1) SP2/0 Cell Lysate |
|---|---|
| Application Notes | NFAT5 antibody can be used for detection of NFAT5 by ELISA at 1:62500. NFAT5 antibody can be used for detection of NFAT5 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFAT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
