Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | NFIA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFIA, CTF, NF1-A, NFI-A, NFI-L, NF-I/A |
| Product description | Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.1) Fazi, F., et al., (2005) Cell 123 (5), 819-831. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFIA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFIA antibody can be used for detection of NFIA by ELISA at 1:1562500. NFIA antibody can be used for detection of NFIA by western blot at 2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFIA |
|---|---|
| Alternative Names | NFIA CTF NF1-A NFI-A NFI-L NF-I/A |
| Molecular Weight(MW) | 55 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NFIA antibody can be used for detection of NFIA by ELISA at 1:1562500. NFIA antibody can be used for detection of NFIA by western blot at 2 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFIA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
