Application
Antibody used in WB on Human Jurkat 0.3125 ug/ml.| Product Name | NFS1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFS1, IscS, NIFS, HUSSY-08 |
| Product description | Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. Two transcript variants encoding two different isoforms have been found for this gene.1) Stanchi, F., et al., (2001) Yeast 18 (1), 69-80. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFS1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| NFS1 antibody can be used for detection of NFS1 by ELISA at 1:312500. NFS1 antibody can be used for detection of NFS1 by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NFS1 |
|---|---|
| Alternative Names | NFS1 IscS NIFS HUSSY-08 |
| Molecular Weight(MW) | 50 kDa |
Application
Antibody used in WB on Human Jurkat 0.3125 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NFS1 antibody can be used for detection of NFS1 by ELISA at 1:312500. NFS1 antibody can be used for detection of NFS1 by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
