Application
Immunofluorescence of NGN2 in human brain tissue with NGN2 antibody at 20 μg/mL.Application
Western blot analysis of NGN2 in mouse small intestine tissue lysate with NGN2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NGN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NGN2 Antibody: NGN2, Atoh4, ngn-2, Math4A, bHLHa8, ATOH4, BHLHA8, NGN2, Neurogenin-2, Class A basic helix-loop-helix protein 8, NGN-2 |
| Product description | NGN2 Antibody: Neurogenin-2 (NGN2) is a neural-specific basic helix-loop-helix (bHLH) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries1) Sommer L, Ma Q, and Anderson DJ. Neurogenins, a novel family of atonal-related bHLH transcription factors, a putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS. Mol. Cell Neurosci. 1996; 8:221-41. |
| Immunogen | Rabbit polyclonal NGN2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human NGN2. The immunogen is located within the last 50 amino acids of NGN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| NGN2 antibody can be used for detection of NGN2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NEUROG2 |
|---|---|
| Alternative Names | NGN2 Antibody: NGN2 Atoh4 ngn-2 Math4A bHLHa8 ATOH4 BHLHA8 NGN2 Neurogenin-2 Class A basic helix-loop-helix protein 8 NGN-2 |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | NGN2 antibody is predicted to not cross-react with neurogenin 1. |
Application
Immunofluorescence of NGN2 in human brain tissue with NGN2 antibody at 20 μg/mL.
Application
Western blot analysis of NGN2 in mouse small intestine tissue lysate with NGN2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1408 - Mouse Small Intestine Tissue Lysate |
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| Application Notes | NGN2 antibody can be used for detection of NGN2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NGN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NGN2 Antibody is supplied in PBS containing 0.02% sodium azide. |
