The helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC, LYL1, E2A, and SCLThe helix-loop-helix (HLH) proteins are a family of putative transcription factors, some of which have been shown to play an important role in growth and development of a wide variety of tissues and species. Four members of this family have been clearly implicated in tumorigenesis via their involvement in chromosomal translocations in lymphoid tumors: MYC (MIM 190080), LYL1 (MIM 151440), E2A (MIM 147141), and SCL (MIM 187040).1) Manetopoulos, C., (2003) Biochem. Biophys. Res. Commun. 307 (4), 891-899.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NHLH1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NHLH1 antibody can be used for detection of NHLH1 by ELISA at 1:1562500. NHLH1 antibody can be used for detection of NHLH1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Heart at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate
Application Notes
NHLH1 antibody can be used for detection of NHLH1 by ELISA at 1:1562500. NHLH1 antibody can be used for detection of NHLH1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NHLH1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.