Application
Immunofluorescence of NIK in 293 cells with NIK antibody at 10 μg/ml.Application
Immunocytochemistry of NIK in 293 cells with NIK antibody at 10 μg/ml.| Product Name | NIK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NIK Antibody: HS, NIK, HSNIK, FTDCR1B, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, HsNIK |
| Product description | NIK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.1) Malinin NL, Boldin MP, Kovalenko AV, et al. MAP3K-related kinase involved in NF-κB induction by TNF, CD95 and IL-1. Nature 1997; 385:540-4. |
| Immunogen | NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK. The immunogen is located within the last 50 amino acids of NIK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K14 |
|---|---|
| Alternative Names | NIK Antibody: HS NIK HSNIK FTDCR1B Mitogen-activated protein kinase kinase kinase 14 NF-kappa-beta-inducing kinase HsNIK |
| Molecular Weight(MW) | Predicted: 104 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (94%) |
Application
Immunofluorescence of NIK in 293 cells with NIK antibody at 10 μg/ml.
Application
Immunocytochemistry of NIK in 293 cells with NIK antibody at 10 μg/ml.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NIK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NIK Antibody is supplied in PBS containing 0.02% sodium azide. |
