Application
Immunofluorescence of nipsnap in human brain tissue with nipsnap antibody at 20 μg/mL.Application
Immunohistochemical staining of human brain tissue using Nipsnap antibody at 2.5 μg/mL.Application
Western blot analysis of NIPSNAP in human brain tissue lysate with NIPSNAP antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | NIPSNAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NIPSNAP Antibody: Protein NipSnap homolog 1, NipSnap1 |
| Product description | NIPSNAP Antibody: NIPSNAP is a member of an evolutionarily well conserved gene family and has a strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. Recent studies have indicated that NIPSNAP is involved in the regulation of the Ca2+-selective transient receptor potential vanilloid channel 6 (TRPV6). NIPSNAP1 associates with TRPV6 at the plasma membrane and inhibits TRPV6 currents. Other studies show that NIPSNAP's expression is reduced in the phenylketonuria (PKU) mouse brain, suggesting that NIPSNAP may play a role in memory.1) Seroussi E, Pan H-Q, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene1998; 212:13-20. |
| Immunogen | NIPSNAP antibody was raised against a 19 amino acid synthetic peptide from near the center of human NIPSNAP. The immunogen is located within amino acids 130 - 180 of NIPSNAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NIPSNAP antibody can be used for the detection of NIPSNAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NIPSNAP1 |
|---|---|
| Alternative Names | NIPSNAP Antibody: Protein NipSnap homolog 1 NipSnap1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of nipsnap in human brain tissue with nipsnap antibody at 20 μg/mL.
Application
Immunohistochemical staining of human brain tissue using Nipsnap antibody at 2.5 μg/mL.
Application
Western blot analysis of NIPSNAP in human brain tissue lysate with NIPSNAP antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | NIPSNAP antibody can be used for the detection of NIPSNAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NIPSNAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NIPSNAP Antibody is supplied in PBS containing 0.02% sodium azide. |
