Application
Immunofluorescence of NIPSNAP3A in Mouse Brain cells with NIPSNAP3A antibody at 5 μg/mL.Application
Immunohistochemistry of NIPSNAP3A in mouse brain tissue with NIPSNAP3A antibody at 2.5 μg/mL.Application
Western blot analysis of NIPSNAP3A in mouse brain tissue lysate with NIPSNAP3A antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | NIPSNAP3A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NIPSNAP3A Antibody: TASSC, HSPC299, NIPSNAP4, Protein NipSnap homolog 3A, Protein NipSnap homolog 4, NipSnap3A |
| Product description | NIPSNAP3A Antibody: The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP3A protein is associated with plasma membrane and partially localized in rafts. NIPSNAP proteins have been suggested to be important in vesicular transport.1) Seroussi E, Pan HQ, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene1998; 212:13-20. |
| Immunogen | NIPSNAP3A antibody was raised against a 16 amino acid synthetic peptide near the center of human NIPSNAP3A. The immunogen is located within amino acids 40 - 90 of NIPSNAP3A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NIPSNAP3A antibody can be used for detection of NIPSNAP3A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NIPSNAP3A |
|---|---|
| Alternative Names | NIPSNAP3A Antibody: TASSC HSPC299 NIPSNAP4 Protein NipSnap homolog 3A Protein NipSnap homolog 4 NipSnap3A |
| Tissue Specificity | At least two isoforms of NIPSNAP3A are known to exist. NIPSNAP3A antibody is predicted to not cross-react with any other members of the NIPSNAP protein family. |
Application
Immunofluorescence of NIPSNAP3A in Mouse Brain cells with NIPSNAP3A antibody at 5 μg/mL.
Application
Immunohistochemistry of NIPSNAP3A in mouse brain tissue with NIPSNAP3A antibody at 2.5 μg/mL.
Application
Western blot analysis of NIPSNAP3A in mouse brain tissue lysate with NIPSNAP3A antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | NIPSNAP3A antibody can be used for detection of NIPSNAP3A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NIPSNAP3A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NIPSNAP3A Antibody is supplied in PBS containing 0.02% sodium azide. |
