Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lysates/proteins at 20 ug per lane.| Product Name | NISCH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nischarin, Imidazoline receptor 1, I-1, IR1, Imidazoline receptor antisera-selected protein, hIRAS, Imidazoline-1 receptor, I1R, Imidazoline-1 receptor candidate protein, I-1 receptor candidate protein, I1R candidate protein, NISCH, IRAS, KIAA0975 |
| Product description | Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (By similarity). Inhibits lamellipodia formation, when overexpressed (By similarity). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.1) Piletz J.E.,et al.DNA Cell Biol. 19:319-329(2000). |
| Immunogen | This NISCH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-38 amino acids from human NISCH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NISCH |
|---|---|
| Alternative Names | Nischarin Imidazoline receptor 1 I-1 IR1 Imidazoline receptor antisera-selected protein hIRAS Imidazoline-1 receptor I1R Imidazoline-1 receptor candidate protein I-1 receptor candidate protein I1R candidate protein NISCH IRAS KIAA0975 |
| Molecular Weight(MW) | 167 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: mouse brain lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
