In the mouse, Nkd is a Dishevelled -binding protein that functions as a negative regulator of the Wnt-beta-catenin -Tcf signaling pathway.In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.1) Yan, D., (2001) Proc. Natl. Acad. Sci. U.S.A. 98 (26), 14973-14978.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NKD1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NKD1 antibody can be used for detection of NKD1 by ELISA at 1:62500. NKD1 antibody can be used for detection of NKD1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
NKD1 antibody can be used for detection of NKD1 by ELISA at 1:62500. NKD1 antibody can be used for detection of NKD1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NKD1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.