Application
Immunohistochemistry of NKX2-6 in human spleen tissue with NKX2-6 antibody at 5 μg/mL.Application
Immunofluorescence of NKX-6 in human spleen tissue with NKX2-6 antibody at 20 μg/mL.Application
Western blot analysis of NKX2-6 in mouse heart tissue lysate with NKX2-6 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | NKX2-6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NKX2-6 Antibody: CSX2, NKX2F, NKX4-2, Homeobox protein NK-2 homolog F |
| Product description | NKX2-6 Antibody: NKX2-6 (NK2 homeobox 6) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-6 is a vertebrate homolog of Drosophila homeobox-containing protein called 'tinman', which has been shown to be essential for development of the heart-like dorsal vessel. In conjunction with related gene, NKX2-5, this gene may play a role in both pharyngeal and cardiac embryonic development. Mutations in this gene are thought to be a cause for some congenital heart abnormalities.1) Copeland NG, Jenkins NA, and Harvey RP. The murine homeobox genes Nkx2.3 and Nkx2.6 are located on chromosomes 19 and 14, respectively. Genomics 1994; 22:655-6. |
| Immunogen | NKX2-6 antibody was raised against a 15 amino acid synthetic peptide near the center of human NKX2-6. The immunogen is located within amino acids 170 - 220 of NKX2-6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NKX2-6 antibody can be used for detection of NKX2-6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NKX2-6 |
|---|---|
| Alternative Names | NKX2-6 Antibody: CSX2 NKX2F NKX4-2 Homeobox protein NK-2 homolog F |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | NKX2-6 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-6 are known to exist; this antibody will detect both isoforms |
| Entrez Gene | 137814 |
|---|---|
| Protein Accession | NP_001129743 |
Application
Immunohistochemistry of NKX2-6 in human spleen tissue with NKX2-6 antibody at 5 μg/mL.
Application
Immunofluorescence of NKX-6 in human spleen tissue with NKX2-6 antibody at 20 μg/mL.
Application
Western blot analysis of NKX2-6 in mouse heart tissue lysate with NKX2-6 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | NKX2-6 antibody can be used for detection of NKX2-6 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NKX2-6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NKX2-6 Antibody is supplied in PBS containing 0.02% sodium azide. |
